Journal: International Journal of Nanomedicine

Article Title: Graphene Oxide Quantum Dots-Preactivated Dental Pulp Stem Cells/GelMA Facilitates Mitophagy-Regulated Bone Regeneration

doi: 10.2147/IJN.S480979

Figure Lengend Snippet: Characterization of GOQDs. ( A ) HRTEM image of the GOQDs dispersion (1 mg/mL) (scale bar of the left photo = 50 nm; scale bar of the right photo = 2 nm). ( B ) Histogram of the particle size distribution obtained by analyzing HRTEM images. ( C ) An AFM image showing the height of GOQDs (scale bar = 500 μm). ( D )The height profile at the positions indicated by the white line. ( E ) Three-dimensional reconstructed AFM image of GOQDs. ( F ) PL spectrum of GOQDs. The lines in different colors indicate the different excitation light at various wavelengths, and the abscissa was the wavelength of the emitted light. ( G ) Full-spectrum XPS image of GOQDs. ( H ) Fine-spectrum XPS scans for oxygen (O 1s). ( I ) Fine-spectrum XPS scans for carbon (C 1s).

Article Snippet: GOQDs dispersion (1 mg/mL; XF074-1) was purchased from Nanjing XFNANO Materials Tech Co., Ltd (China).

Techniques: Dispersion

Journal: International Journal of Nanomedicine

Article Title: Graphene Oxide Quantum Dots-Preactivated Dental Pulp Stem Cells/GelMA Facilitates Mitophagy-Regulated Bone Regeneration

doi: 10.2147/IJN.S480979

Figure Lengend Snippet: Effect of GOQDs on the viability, osteogenic differentiation, and migration of DPSCs in vitro. ( A ) CCK-8 analysis showing the effect of different concentrations of GOQDs on the viability of DPSCs (n = 3). (B‒H) Effects of different concentrations of GOQDs on the osteogenic differentiation of DPSCs. ( B ) Representative ALP staining images (scale bar = 500 μm). ( C ) ALP activity assay (n = 3). ( D ) Representative ARS staining images (scale bar = 500 μm). ( E ) Quantitative analysis of mineralized nodules stained with ARS (n = 3) ( F ) Representative Western blot images showing the effect of GOQDs on osteogenic marker molecules in DPSCs. ( G ) The relative expression levels of Col-1 in ( F ) (n = 3). ( H ) The relative expression levels of OPN in ( F ) (n = 3). ( I ) Representative images of DPSCs at 3 h and 24 h after scratching (scale bar = 200 μm). ( J ) Migration indices of DPSCs treated with different concentrations of GOQDs (n = 3).

Article Snippet: GOQDs dispersion (1 mg/mL; XF074-1) was purchased from Nanjing XFNANO Materials Tech Co., Ltd (China).

Techniques: Migration, In Vitro, CCK-8 Assay, Staining, ALP Activity Assay, Western Blot, Marker, Expressing

Journal: International Journal of Nanomedicine

Article Title: Graphene Oxide Quantum Dots-Preactivated Dental Pulp Stem Cells/GelMA Facilitates Mitophagy-Regulated Bone Regeneration

doi: 10.2147/IJN.S480979

Figure Lengend Snippet: In vitro growth and differentiation capacity of DPSCs in GelMA. ( A ) Representative SEM images of different concentrations of GelMA (scale bar = 20 μm). ( B, C ) Average pore size and porosity of the hydrogels. ( D ) CCK-8 assay of DPSCs cultured in different concentrations of GelMA (n = 3). ( E ) Representative Calcein AM/PI fluorescence images of DPSCs/hydrogel complexes incubated with GM or GM containing 200 nM STS (GM+STS group) (scale bar = 200 μm). The GM+STS group served as a positive control to evaluate whether cells encapsulated in GelMA had full access to the drugs in the medium. The live cells were stained with Calcein AM (green), and the dead cells were stained with PI (red). ( F ) Representative ALP staining images of DPSCs/hydrogel complexes (scale bar = 500 μm). Neither the culture medium nor the hydrogel contained 5 μg/mL GOQDs in the DPSCs/Gel group, whereas both the culture medium and hydrogel contained 5 μg/mL GOQDs in the GOQDs/DPSCs/Gel group.

Article Snippet: GOQDs dispersion (1 mg/mL; XF074-1) was purchased from Nanjing XFNANO Materials Tech Co., Ltd (China).

Techniques: In Vitro, Pore Size, CCK-8 Assay, Cell Culture, Fluorescence, Incubation, Positive Control, Staining

Journal: International Journal of Nanomedicine

Article Title: Graphene Oxide Quantum Dots-Preactivated Dental Pulp Stem Cells/GelMA Facilitates Mitophagy-Regulated Bone Regeneration

doi: 10.2147/IJN.S480979

Figure Lengend Snippet: GOQDs/DPSCs/Gel promoted bone regeneration in vivo. ( A ) Grouping and flow of animal experiments (created with Biorender.com). ( B ) Three-dimensional reconstructed micro-CT images. ( C ) New bone volume at the defect site was calculated from micro-CT data (n = 6). ( D ) The surface density of new bone at the defect site was calculated from micro-CT data (n = 6). ( E ) Representative HE staining images (scale bar of the upper panel = 1 mm; scale bar of the lower panel = 500 μm). ( F ) The semiquantitative analysis of the new bone area in HE staining images (n = 6). ( G ) Representative Masson’s trichrome staining images (scale bar of the upper panel = 1 mm; scale bar of the lower panel = 500 μm). ( H ) The semiquantitative analysis of the area of the newly formed immature collagen in Masson’s trichrome staining images (n = 6). ( I ) Representative images of immunohistochemical staining for OCN (scale bar = 500 μm). ( J ) The semiquantitative analysis of the OCN-positive area in the immunohistochemical staining for OCN images (n = 6). NB indicates the newly formed bone (NB). HB indicates the host’s bone (HB).

Article Snippet: GOQDs dispersion (1 mg/mL; XF074-1) was purchased from Nanjing XFNANO Materials Tech Co., Ltd (China).

Techniques: In Vivo, Micro-CT, Staining, Immunohistochemical staining

Journal: International Journal of Nanomedicine

Article Title: Graphene Oxide Quantum Dots-Preactivated Dental Pulp Stem Cells/GelMA Facilitates Mitophagy-Regulated Bone Regeneration

doi: 10.2147/IJN.S480979

Figure Lengend Snippet: GOQDs promoted mitophagy in DPSCs. ( A ) Representative TEM images of DPSCs induced with or without GOQDs (scale bar of the upper panel = 1 μm; scale bar of the lower panel = 250 nm). The Orange arrowheads indicate the mitochondria engulfed by lysosomes. ( B ) Representative JC-1 staining images showing the influence of GOQDs on the MMP of DPSCs (scale bar = 100 μm). ( C ) Ratios of red/green fluorescence intensity were calculated to determine the MMP (n = 3). ( D ) Colocalization of mitochondria (green) and lysosomes (red) in DPSCs treated with or without GOQDs (scale bar = 25 μm). The colocalization scatterplot was obtained by analyzing the merge image using the Color2 plugin of ImageJ software. ( E ) Semiquantitative analysis of colocalization images (n = 3). ( F ) Representative Western blot images showing the effect of GOQDs induction on the expression of the mitophagy markers. ( G ) The relative expression levels of p62 in ( F ) (n = 3). ( H ) The relative expression levels of PINK1 in ( F ) (n = 3). ( I ) The relative expression levels of PRKN in ( F ) (n = 3). ( J ) The ratios of LC3II/I expression levels in ( F ) (n = 3).

Article Snippet: GOQDs dispersion (1 mg/mL; XF074-1) was purchased from Nanjing XFNANO Materials Tech Co., Ltd (China).

Techniques: Staining, Fluorescence, Software, Western Blot, Expressing

Journal: International Journal of Nanomedicine

Article Title: Graphene Oxide Quantum Dots-Preactivated Dental Pulp Stem Cells/GelMA Facilitates Mitophagy-Regulated Bone Regeneration

doi: 10.2147/IJN.S480979

Figure Lengend Snippet: Mitophagy inhibition weakened GOQDs-induced osteogenic differentiation in DPSCs. ( A ) Representative JC-1 staining images showing the effect of CsA on the MMP of DPSCs (scale bar = 100 μm). ( B ) The semiquantitative analysis of JC-1 staining images (n = 3). The ratio of red/green fluorescence intensity indicates the MMP. ( C ) Representative fluorescence images showing the colocalization of mitochondria (green) and lysosomes (red) (scale bar = 25 μm) and the colocalization scatterplot, which was obtained by analyzing the merge image using the Color2 plugin of ImageJ software. ( D ) The semiquantitative analysis of the fluorescence colocalization images (n = 3). ( E ) Representative Western blot images showing the effect of CsA on the expression of mitophagy markers. ( F ) The relative expression levels of PINK1 in ( E ) (n = 3). ( G ) The relative expression levels of PRKN in ( E ) (n = 3). ( H ) The relative expression levels of p62 in ( E ) (n = 3). ( I ) The ratios of LC3 II/I expression levels in ( E ) (n = 3). ( J ) Representative Western blot images showing the effect of mitophagy inhibition on the expression of osteogenic transcription factors (n = 3). ( K ) The relative expression levels of Runx2 in ( J ) (n = 3). ( L ) The relative expression levels of Osx in ( J ) (n = 3). ( M ) Representative ALP staining images showing the effect of mitophagic inhibition on the ALP expression in DPSCs (scale bar = 500 μm).

Article Snippet: GOQDs dispersion (1 mg/mL; XF074-1) was purchased from Nanjing XFNANO Materials Tech Co., Ltd (China).

Techniques: Inhibition, Staining, Fluorescence, Software, Western Blot, Expressing

Journal: International Journal of Nanomedicine

Article Title: Graphene Oxide Quantum Dots-Preactivated Dental Pulp Stem Cells/GelMA Facilitates Mitophagy-Regulated Bone Regeneration

doi: 10.2147/IJN.S480979

Figure Lengend Snippet: Summary of DPSCs activated by GOQDs repair rat calvarial bone defect via promoting mitophagy-related osteogenic differentiation. GOQDs/DPSCs/GelMA hydrogel composites are incubated in GOQDs-containing medium in vitro for eight days and then implanted into calvarial bone defects. The GOQDs/DPSCs/GelMA composites effectively repair the bone defects. The underlying mechanism is revealed that GOQDs induce mitophagy in DPSCs, as evidenced by a decrease in MMP, up-regulation of the expression of PRKN, PINK1, LC3, and P62, and an increase in the co-localization of mitochondria and lysosomes. Then, the mitophagy-regulated osteogenic differentiation of DPSCs is promoted by GOQDs (created with Biorender.com).

Article Snippet: GOQDs dispersion (1 mg/mL; XF074-1) was purchased from Nanjing XFNANO Materials Tech Co., Ltd (China).

Techniques: Incubation, In Vitro, Expressing

Journal: Nano Convergence

Article Title: Investigating composite electrode materials of metal oxides for advanced energy storage applications

doi: 10.1186/s40580-024-00437-2

Figure Lengend Snippet: Metal oxides and its composites synthesis method, morphology and specific capacitance/capacity comparison for SCs

Article Snippet: [ , , , ] Ferrite quantum dots (QD) on graphene heterostructures using a simple biomimetic mineralization synthetic method was also introduced, as shown in Fig. (c) [ ].

Techniques: Comparison, Aerosol, Suction Filtration, Modification, Polymer

Journal: Small Science

Article Title: Emerging Multifunctional Carbon‐Nanomaterial‐Based Biosensors for Cancer Diagnosis

doi: 10.1002/smsc.202300221

Figure Lengend Snippet: Summary of ECL biosensors used to detect cancer biomarkers

Article Snippet: In a separate investigation, Sun and his team integrated graphene QDs with molybdenum disulfide (MoS2‐GQDs) to design an ECL biosensor with an “on/off” switch, enabling the detection of specific DNA sequences.

Techniques:

Journal: Small Science

Article Title: Emerging Multifunctional Carbon‐Nanomaterial‐Based Biosensors for Cancer Diagnosis

doi: 10.1002/smsc.202300221

Figure Lengend Snippet: Electrochemical biosensors produced for cancer biomarkers detection

Article Snippet: In a separate investigation, Sun and his team integrated graphene QDs with molybdenum disulfide (MoS2‐GQDs) to design an ECL biosensor with an “on/off” switch, enabling the detection of specific DNA sequences.

Techniques: Produced

Journal: Small Science

Article Title: Emerging Multifunctional Carbon‐Nanomaterial‐Based Biosensors for Cancer Diagnosis

doi: 10.1002/smsc.202300221

Figure Lengend Snippet: Schematic diagram depicting the operational mechanism of the fluorescent biosensor for detecting small cell lung cancer biomarkers by neuron‐specific enolase antibodies (anti‐NSE)/amine nitrogen‐doped graphene quantum dots with AuNP. Reproduced with permission. [ ²⁸² ] Copyright 2020, American Chemical Society.

Article Snippet: In a separate investigation, Sun and his team integrated graphene QDs with molybdenum disulfide (MoS2‐GQDs) to design an ECL biosensor with an “on/off” switch, enabling the detection of specific DNA sequences.

Techniques:

Journal: Small Science

Article Title: Emerging Multifunctional Carbon‐Nanomaterial‐Based Biosensors for Cancer Diagnosis

doi: 10.1002/smsc.202300221

Figure Lengend Snippet: Summary of fluorescence biosensors for cancer biomarker detection

Article Snippet: In a separate investigation, Sun and his team integrated graphene QDs with molybdenum disulfide (MoS2‐GQDs) to design an ECL biosensor with an “on/off” switch, enabling the detection of specific DNA sequences.

Techniques: Fluorescence, Biomarker Discovery

Journal: Small Science

Article Title: Emerging Multifunctional Carbon‐Nanomaterial‐Based Biosensors for Cancer Diagnosis

doi: 10.1002/smsc.202300221

Figure Lengend Snippet: a) Schematic diagram of the SPR biosensor equipped with the GO‐linking layer. b) SPR profiles of the unmodified gold sensor chip, monolayer graphene chip, and GO chip. c) The sensitivity to alterations in the refractive index of the respective SPR biosensor chips. a–c) Reproduced with permission. [ ³⁰⁵ ] Copyright 2015, American Chemical Society. d) Schematic illustration of QCM‐based immunosensor. e) QCM curves (frequency vs time) of (i) anti‐IL‐6‐Ab1/AuNPs/S‐GQD/QCM, (ii) IL‐6/anti‐IL‐6‐Ab1/AuNPs/S‐GQD/QCM, and (iii) anti‐IL‐6‐Ab2/ASP‐h‐ZnS‐CdS NC conjugated to IL‐6/anti‐IL‐6‐Ab1/AuNPs/S‐GQD/QCM. f) Concentration effect on QCM frequency. The inset shows the corresponding calibration curve. d–f) Reproduced with permission. [ ³²¹ ] Copyright 2021, Elsevier.

Article Snippet: In a separate investigation, Sun and his team integrated graphene QDs with molybdenum disulfide (MoS2‐GQDs) to design an ECL biosensor with an “on/off” switch, enabling the detection of specific DNA sequences.

Techniques: Refractive Index, Concentration Assay

Journal: Small Science

Article Title: Emerging Multifunctional Carbon‐Nanomaterial‐Based Biosensors for Cancer Diagnosis

doi: 10.1002/smsc.202300221

Figure Lengend Snippet: Summary of SPR and QCM biosensors for cancer biomarker detection

Article Snippet: In a separate investigation, Sun and his team integrated graphene QDs with molybdenum disulfide (MoS2‐GQDs) to design an ECL biosensor with an “on/off” switch, enabling the detection of specific DNA sequences.

Techniques: Biomarker Discovery

Journal: Advanced Science

Article Title: Nature‐Inspired 3D Spiral Grass Structured Graphene Quantum Dots/MXene Nanohybrids with Exceptional Photothermal‐Driven Pseudo‐Capacitance Improvement

doi: 10.1002/advs.202204086

Figure Lengend Snippet: Electrochemical performance of the biomimetic structural GQDs/MXene photothermal SCs. CV profiles of the GQDs/MXene‐720° SC at different scan rates a) in the dark and b) under 1 solar illumination. c) Comparative CV curves at 10 mV s −1 of the device in the dark and under 1 solar illumination. d) GCD curves of the SC at various current densities in the dark and under 1 solar illumination. e) Rate capability and f) Nyquist plots of the device in the dark and under 1 solar illumination. g) Ragone plot of the biomimetic structural GQDs/MXene photothermal SC in comparison with other recently reported 3D‐printed or MXene‐based SCs. h) Cycling performance of the device at 0.2 A cm −2 in the dark and under 1 solar illumination.

Article Snippet: A biomimetic structural graphene quantum dots/MXene photothermal supercapacitor inspired by the light‐trapping effect of wide‐leaf spiral grass is developed.

Techniques: Comparison

Journal: Advanced Science

Article Title: Nature‐Inspired 3D Spiral Grass Structured Graphene Quantum Dots/MXene Nanohybrids with Exceptional Photothermal‐Driven Pseudo‐Capacitance Improvement

doi: 10.1002/advs.202204086

Figure Lengend Snippet: Electrochemical performance of the biomimetic structural GQDs/MXene‐720° photothermal SC under sunlight with different power densities. a) Surface temperature, b) CV curves at 100 mV s −1 , c) GCD curves at 100 mA cm −2 , and d) Nyquist plots of the symmetric GQDs/MXene‐720° SC under solar illumination at different power densities. e) Schematic illustration of the structure‐enhanced solar thermal‐driven capacitance enhancement of the biomimetic structural GQDs/MXene photothermal SC.

Article Snippet: A biomimetic structural graphene quantum dots/MXene photothermal supercapacitor inspired by the light‐trapping effect of wide‐leaf spiral grass is developed.

Techniques:

Journal: RSC Advances

Article Title: Improvement in luminescent properties and thermo-optical conversion mechanism of Na 2 SiF 6 :Mn 4+ ,K + @GQDs

doi: 10.1039/d1ra02449c

Figure Lengend Snippet: XRD patterns of the three samples, (i) NSF:0.05Mn 4+ , (ii) NSF:0.05Mn 4+ ,0.04K + , (iii) NSF:0.05Mn 4+ ,0.04K + @GQDs 8 mg mol −1 : (a) full XRD patterns. (c) and (d) Crystal structure of sample (ii).

Article Snippet: Cl-Containing graphene quantum dots (CGQDs) were purchased from Nanjing XFNANO Materials Tech Co. Ltd, China, and used without further purification.

Techniques:

Journal: RSC Advances

Article Title: Improvement in luminescent properties and thermo-optical conversion mechanism of Na 2 SiF 6 :Mn 4+ ,K + @GQDs

doi: 10.1039/d1ra02449c

Figure Lengend Snippet: Comparison of the negative thermal quenching of some samples a

Article Snippet: Cl-Containing graphene quantum dots (CGQDs) were purchased from Nanjing XFNANO Materials Tech Co. Ltd, China, and used without further purification.

Techniques: Comparison

Journal: RSC Advances

Article Title: Improvement in luminescent properties and thermo-optical conversion mechanism of Na 2 SiF 6 :Mn 4+ ,K + @GQDs

doi: 10.1039/d1ra02449c

Figure Lengend Snippet: SEM images, EDS, XPS and FTIR spectra of two samples, (ii) NSF:0.05Mn 4+ ,0.04K + and (iii) NSF:0.05Mn 4+ ,0.04K + @GQDs 8 mg mol −1 : (a) and (b) SEM images of samples (ii) and (iii). (c) and (d) EDS spectra. (e) XPS spectra. (f) and (g) High-resolution XPS of Mn 2p. (h) FTIR spectra.

Article Snippet: Cl-Containing graphene quantum dots (CGQDs) were purchased from Nanjing XFNANO Materials Tech Co. Ltd, China, and used without further purification.

Techniques:

Journal: RSC Advances

Article Title: Improvement in luminescent properties and thermo-optical conversion mechanism of Na 2 SiF 6 :Mn 4+ ,K + @GQDs

doi: 10.1039/d1ra02449c

Figure Lengend Snippet: Luminescent properties of four samples, (0) NSF@GQDs 8 mg mol −1 , (i) NSF:0.05Mn 4+ , (ii) NSF:0.05Mn 4+ ,0.04K + , and (iii) NSF:0.05Mn 4+ ,0.04K + @GQDs 8 mg mol −1 : (a) PLE spectra, (b) PL spectra, (c) diagram of energy level transition induced by GQDs, (d) decay curves, and (e) CIE chromaticity diagrams.

Article Snippet: Cl-Containing graphene quantum dots (CGQDs) were purchased from Nanjing XFNANO Materials Tech Co. Ltd, China, and used without further purification.

Techniques:

Journal: RSC Advances

Article Title: Improvement in luminescent properties and thermo-optical conversion mechanism of Na 2 SiF 6 :Mn 4+ ,K + @GQDs

doi: 10.1039/d1ra02449c

Figure Lengend Snippet: Luminescent properties of NSF: y Mn 4+ , NSF:0.05Mn 4+ , x K + and NSF:0.05Mn 4+ ,0.04K + @GQDs z mg mol −1 : (a), (c) and (d) PL spectra and (b) related line type of multipolar interaction.

Article Snippet: Cl-Containing graphene quantum dots (CGQDs) were purchased from Nanjing XFNANO Materials Tech Co. Ltd, China, and used without further purification.

Techniques:

Journal: RSC Advances

Article Title: Improvement in luminescent properties and thermo-optical conversion mechanism of Na 2 SiF 6 :Mn 4+ ,K + @GQDs

doi: 10.1039/d1ra02449c

Figure Lengend Snippet: PL performances of the three samples at different temperatures, (i) NSF:0.05Mn 4+ , (ii) NSF:0.05Mn 4+ ,0.04K + , (iii) NSF:0.05Mn 4+ ,0.04K + @GQDs 8 mg mol −1 : (a), (b), and (c) PL spectra. (d) Integrated intensity curve. (e) Integrated intensity curves of Stoke and anti-Stoke peaks. (f) Chromaticity shift of sample (iii). (g) E a curves. (h) CIE color coordinates of sample (iii).

Article Snippet: Cl-Containing graphene quantum dots (CGQDs) were purchased from Nanjing XFNANO Materials Tech Co. Ltd, China, and used without further purification.

Techniques:

Journal: RSC Advances

Article Title: Improvement in luminescent properties and thermo-optical conversion mechanism of Na 2 SiF 6 :Mn 4+ ,K + @GQDs

doi: 10.1039/d1ra02449c

Figure Lengend Snippet: TG of sample (iii) (NSF:0.05Mn 4+ ,0.04K + @GQDs 8 mg mol −1 ).

Article Snippet: Cl-Containing graphene quantum dots (CGQDs) were purchased from Nanjing XFNANO Materials Tech Co. Ltd, China, and used without further purification.

Techniques:

Journal: RSC Advances

Article Title: Improvement in luminescent properties and thermo-optical conversion mechanism of Na 2 SiF 6 :Mn 4+ ,K + @GQDs

doi: 10.1039/d1ra02449c

Figure Lengend Snippet: Water resistance test of two samples: (ii) NSF:0.05Mn 4+ ,0.04K + and (iii) NSF:0.05Mn 4+ ,0.04K + @GQDs 8 mg mol −1 : (a) photographs under natural light and (b) PL integrated intensities with different soaking times in water.

Article Snippet: Cl-Containing graphene quantum dots (CGQDs) were purchased from Nanjing XFNANO Materials Tech Co. Ltd, China, and used without further purification.

Techniques:

Journal: RSC Advances

Article Title: Improvement in luminescent properties and thermo-optical conversion mechanism of Na 2 SiF 6 :Mn 4+ ,K + @GQDs

doi: 10.1039/d1ra02449c

Figure Lengend Snippet: Properties of prototype WLEDs fabricated using the mixed phosphors of NSF:0.05Mn 4+ ,0.04K + @GQDs 8 mg mol −1 and YAG:Ce 3+ based on an InGaN chip under a 20 mA drive current: (a) electroluminescence spectrum, (b) CIE chromaticity diagram and (c) luminescent photo.

Article Snippet: Cl-Containing graphene quantum dots (CGQDs) were purchased from Nanjing XFNANO Materials Tech Co. Ltd, China, and used without further purification.

Techniques: